M.G. MONA*^#, A.I. EL-AWADY*, M.S. TALAAT**, O.S. DESOUKY***, N.S. EL-HANSI***

* Biophysics and Laser Science Unit, Research Institute of Ophthalmology, Giza, Egypt
**Biophysics Branch, Physics department, Faculty of Science, Ain Shams University, Cairo, Egypt
*** Biophysics Laboratory, Radiation Physics Department, National Center for Radiation Research and Technology (NCRRT), EAEA, POB 29 Madinat Nasr Cairo, Egypt

The aim of the present study is to investigate the effect of blue light (403 nm) on electrorentinogram (ERG) and retinal rhodopsin. Also, the possible protective role of grape seed extract (GSE) was tested. Newzealand albino rabbits were used in this study. The rabbits exposed to blue light with and without GSE supplementation for 48 hours, one week, two weeks and 3 weeks respectively. At the end of each period, the electroretinogram (ERG) was recorded. The rabbit were decapitated; the rhodopsin was extracted, malondialdehyde (MDA), total antioxidant capacity (TAC) and fourier transform infrared spectrum (FTIR) of bleached rhodopsin was measured. The data indicates changes in ERG, MDA, TAC and FTIR spectra after blue light exposure. Supplementation of GSE improves the ERG parameters, leads to decrease MDA level and increase the TAC with respect to non supplemented antioxidant groups. It is concluded that long exposure to blue light affects retina and GSE may have protective effects that help in protecting the retina from blue light damage.

Corresponding author’s e-mail: monamg1@yahoo.com

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