LUMINIȚA SEVASTIȚA MUNTEANU*, A. DINU*, CORINA POSEA**, SONIA HERMAN*

*Biophysics Department, ‘Carol Davila” University of Medicine and Pharmaceutics, 8 Eroii Sanitari Blvd, 76241 Bucharest, Romania
**Department of Hematology and Hemobiology, University Hospital, Bucharest, Romania

Abstract. We investigated the effect of insulin on reactive oxygen species (ROS) production by human neutrophils stimulated with opsonized zymosan and incubated for 30 minutes with different insulin concentrations. For measuring ROS production, we used luminol-dependent chemiluminescence assay (CLLD). We observed that the CLLD depends on insulin concentration and this supports the hypothesis that insulin may play a role in ROS production by normal human neutrophils.

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