I.I. DOROBANŢU, LIVIA HĂRĂNGUŞ, M. RADU, ANDREEA IORDAN, DELIA IRINA COROL

„Horia Hulubei” National Institute of R&D for Physics and Nuclear Engineering Bucharest, 407 Atomiştilor Street, P.O. Box MG-6, 077125, Bucharest-Măgurele, Romania

Abstract. The affinity constants, the rate constants and the optimal time to achieve a chemical equilibrium represent the essential immunogenic properties of the antibody-antigen systems required to define optimal conditions for ELISA (Enzyme Linked ImmunoSorbent Assay) analyses. Our paper evaluate the affinity constant of the 8OHdG (8-hydroxy-2-deoxyguanosine) antigen, covalently bounded on the glass tube surface, for alkaline phosphatase labeled anti-8OHdG antibody conjugate in the presence or absence of free 8OHdG antigen

Corresponding author’s e-mail: mradu@nipne.ro

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